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A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1Δ and ypt6Δ Strains

机译：酵母菌arl1Δ和ypt6Δ菌株自噬缺陷的高复制抑制子筛选

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In Saccharomyces cerevisiae, and , two small GTP-binding proteins that regulate membrane traffic in the secretory and endocytic pathways, are also necessary for autophagy. To gain information about potential partners of and specifically in autophagy, we carried out a high copy number suppressor screen to identify genes that when overexpressed suppress the rapamycin sensitivity phenotype of Δ and Δ strains at 37°. From the screen results, we selected , , , , , , and , either membrane traffic or autophagy regulators, to further test whether they can suppress the specific autophagy defects of Δ and Δ strains. As a result, we identified , href="http://www.yeastgenome.org/locus/S000003573/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">SNX4, and href="http://www.yeastgenome.org/locus/S000003619/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">TAX4 to be specific suppressors for the href="http://www.yeastgenome.org/locus/S000000368/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">arl1Δ strain, and href="http://www.yeastgenome.org/locus/S000002637/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">IVY1 and href="http://www.yeastgenome.org/locus/S000006070/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">ATG5 for the href="http://www.yeastgenome.org/locus/S000004252/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">ypt6Δ strain. Through this screen, we were able to confirm several membrane traffic and autophagy regulators that have novel relationships with href="http://www.yeastgenome.org/locus/S000000368/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Arl1 and href="http://www.yeastgenome.org/locus/S000004252/overview" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=5295583&issue-id=287092&journal-id=1684&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Ypt6 during autophagy.

机译：在酿酒酵母中，自噬还需要两个小的GTP结合蛋白，它们调节分泌和内吞途径中的膜运输。为了获得有关自噬以及特别是自噬的潜在伴侣的信息，我们进行了高拷贝数抑制子筛选，以鉴定在过表达时能抑制37度Δ和Δ菌株的雷帕霉素敏感性表型的基因。从筛选结果中，我们选择，，，，，和膜运输或自噬调节剂，以进一步测试它们是否可以抑制Δ和Δ菌株的特定自噬缺陷。结果，我们确定了href =“ http://www.yeastgenome.org/locus/S000003573/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> SNX4 和href =” http://www.yeastgenome.org/locus/S000003619/概述” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> TAX4 < / a>作为href =“ http://www.yeastgenome.org/locus/S000000368/overview” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 5295583＆issue- id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> arl1 Δ株和 href =” http：//www.yeastgenome .org / locus / S000002637 / overview“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> IVY1 和 href =” http://www.yeastgenome.org/locus/ S000006070 / overview“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> href="http://www.yeastgenome.org/locus/S000004252/overview" data-ga-action="click_feat_suppl"ref="reftype=extlink&article的ATG5 -id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> ypt6 Δ菌株。通过此屏幕，我们能够确认与href =“ http://www.yeastgenome.org/locus/S000000368/overview” data-ga-action =“ click_feat_suppl”有新颖关系的几个膜运输和自噬调节剂ref =“ reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> Arl1 和href =“ http： //www.yeastgenome.org/locus/S000004252/overview“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 5295583＆issue-id = 287092＆journal-id = 1684＆FROM = Article％7CFront％20Matter＆TO = External％ 7CLink％7CURI“ target =” _ blank“> Ypt6 在自噬过程中。 展开▼

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期刊名称 G3: GenesGenomesGenetics

作者
Shu Yang; Anne Rosenwald;
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作者单位

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年(卷),期 2017(7),2

年度 2017

页码 333–341

总页数 9

原文格式 PDF

正文语种

中图分类遗传学;

关键词
Saccharomyces cerevisiae ARL1 YPT6 autophagy rapamycin;

机译：酿酒酵母;ARL1;YPT6;自噬;雷帕霉素;

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专利

1. A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1Δ and ypt6Δ Strains [J] . Shu Yang, Anne Rosenwald G3: Genes, Genomes, Genetics . 2017,第2期

机译：酵母菌arl1Δ和ypt6Δ菌株自噬缺陷的高复制抑制子筛选

2. Autophagy in Saccharomyces cerevisiae requires the monomeric GTP-binding proteins, Arl1 and Ypt6 [J] . Yang Shu, Rosenwald Anne G. Autophagy . 2016,第10期

机译：酿酒酵母中的自噬需要，需要单体GTP结合蛋白，ARL1和YPT6

3. sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase. [J] . B M Culley, C S Hoffman, E Apolinario, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1995,第2期

机译：sck1是裂变酵母中cAMP依赖性蛋白激酶途径中缺陷的高拷贝数抑制剂，编码与酿酒酵母SCH9激酶同源的蛋白。

4. Domestication and Screening of Saccharomyces Cerevisiae Strain Resistant to Inhibitors in Lignocellulosic Hydrolysates by Acclimatizing Inhibitory [C] . Shaohua Luo, Yongwen Huang, An Chen, REET 2013 . 2014

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5. Functional Genomic Screens for Novel Metastasis Suppressor Genes in Breast and Prostate Cancer Models [D] . Withers, Henry G. 2018

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6. Autophagy in Saccharomyces cerevisiae requires the monomeric GTP-binding proteins Arl1 and Ypt6 [O] . Shu Yang, Anne G. Rosenwald 2016

机译：酿酒酵母中的自噬需要单体GTP结合蛋白Arl1和Ypt6

7. A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1Δ and ypt6Δ Strains [O] . Shu Yang, Anne Rosenwald 2017

机译：用于酿酒酵母arl1Δ和ypt6Δ菌株中自噬缺陷的高拷贝抑制剂筛选

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1. 一种酿酒酵母菌株及酿酒酵母筛选方法及酵母菌株酿造杨梅酒的工艺 [P] . 中国专利： CN106350462A . 2017-01-25

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A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1Δ and ypt6Δ Strains

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