Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list

摘要

Differential gene expression analysis may discover a set of genes too large to easily investigate, so a means of ranking genes by biological interest level is desired. p values are frequently abused for this purpose. As an alternative, we propose a method of ranking by confidence bounds on the log fold change, based on the previously developed TREAT test. These confidence bounds provide guaranteed false discovery rate and false coverage-statement rate control. When applied to a breast cancer dataset, the top-ranked genes by Topconfects emphasize markedly different biological processes compared to the top-ranked genes by p value.Electronic supplementary materialThe online version of this article (10.1186/s13059-019-1674-7) contains supplementary material, which is available to authorized users.