Genome-wide deletion mutant analysis reveals genes required for respiratory growth mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae

摘要

BackgroundThe mitochondrial respiratory chain produces metabolic energy by oxidative phosphorylation. Biogenesis of the respiratory chain requires the coordinated expression of two genomes: the nuclear genome encoding the vast majority of mitochondrial proteins, and the mitochondrial genome encoding a handful of mitochondrial proteins. The understanding of the molecular processes contributing to respiratory chain assembly and maintenance requires the systematic identification and functional analysis of the genes involved.