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Profiling of microbial community of Odisha hot spring based on metagenomic sequencing

机译:基于宏基因组测序的奥里萨邦温泉微生物群落概况

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摘要

Deulajhari hot spring has diverse temperature and pH range varying from 43 °C to 65 °C and 7.83 to 8.10 respectively. Dense foliage around Deulajhari hot spring contributes to the high total organic carbon content (TOC). In our experiment we took sediment samples from the two Deulajhari hot springs (S1 and S2) out of the cluster having temperature of 43 °C and 55 °C and pH of 7.83 and 7.14 respectively. Sediment samples were analysed using 16S rRNA of V3‐V4 region by amplicon metagenome sequencing. Over 34 phyla were detected in cluster S1 and 32 phyla in cluster S2 at the existing physiochemical parameters temperature 43 °C, pH 7.83, electroconductivity 0.019 dSm− 1, and total organic carbon (TOC) 3.80% for S1 and temperature 55 °C, pH 7.14, electroconductivity 0.019 dSm− 1, and total organic carbon (TOC) 0.97% for S2. Existence of a vast number of unresolved sequences 179 out of 292 in S1 and 186 out of 314 in S2 at the genus level emphasizes the significance of our study. Metagenome sequence information for the both clusters S1 and S2 of Deulajhari is available at NCBI, SRA database with accession number SRX1459732 and SRX1459733 respectively.Direct link to the deposited data:
机译:Deulajhari温泉的温度和pH范围从43°C到65°C和7.83到8.10不等。 Deulajhari温泉周围茂密的树叶有助于提高总有机碳含量(TOC)。在我们的实验中,我们从温度分别为43°C和55°C,pH分别为7.83和7.14的簇中取出了两个Deulajhari温泉(S1和S2)的沉积物样品。通过扩增子基因组测序,使用V3-V4区的16S rRNA分析沉积物样品。在现有的理化参数温度为43°C,pH为7.83,电导率为0.019 dSm −1 ,总有机碳(TOC)为3.80%的情况下,在聚类S1和聚类S2中检测到超过34种门。 S1的温度为55°C,pH为7.14,电导率为0.019 dSm −1 ,S2的总有机碳(TOC)为0.97%。在属水平上,存在大量未解析序列,即S1中292个中的179个以及S2中314个中的186个,这突出了我们研究的重要性。可在NCBI,SRA数据库中分别获得登录号为SRX1459732和SRX1459733的Deulajhari集群S1和S2的元基因组序列信息,直接链接至保存的数据:

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