首页> 美国卫生研究院文献>Genomics Data >De novo transcriptome assembly of two different apricot cultivars
【2h】

De novo transcriptome assembly of two different apricot cultivars

机译:从头转录组的两个不同的杏品种。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Apricot (Prunus armeniaca) belonging to the Prunus species is a popular kind of stone fruit tree. Apricot is native to Armenia and is currently cultivated in many countries with climates adaptable for apricot growth. In general, fresh fruits as well as dried apricot are produced. However, the information associated with genes and genetic markers for apricot is very limited. In this study, we carried out de novo transcriptome assembly for two selected apricot cultivars referred to as Harcot and Ungarische Beste, which are commercially important apricot cultivars in the world, using next generation sequencing. We obtained a total of 9.31 GB and 8.88 GB raw data from Harcot and Ungarische Beste (NCBI accession numbers: SRX1186946 and SRX1186893), respectively. De novo transcriptome assembly using Trinity identified 147,501 and 152,235 transcripts for Harcot and Ungarische Beste, respectively. Next, we identified 113,565 and 126,444 proteins from Harcot and Ungarische Beste using the TransDecoder program. We performed BLASTP against an NCBI non-redundant (nr) dataset to annotate identified proteins. Taken together, we provide transcriptomes of two different apricot cultivars by RNA-Seq.
机译:属于李属的杏(Prunus armeniaca)是一种流行的核果树。杏原产于亚美尼亚,目前在许多国家种植,气候适合杏生长。通常,生产新鲜水果以及杏干。但是,与杏的基因和遗传标记有关的信息非常有限。在这项研究中,我们使用下一代测序技术,对两个选定的称为Harcot和Ungarische Beste的杏品种进行了从头转录组组装,这两个品种在世界上是商业上重要的杏品种。我们分别从Harcot和Ungarische Beste(NCBI登录号:SRX1186946和SRX1186893)获得了总计9.31 GB和8.88 GB的原始数据。使用Trinity从头进行转录组组装,分别为Harcot和Ungarische Beste鉴定了147,501和152,235转录本。接下来,我们使用TransDecoder程序从Harcot和Ungarische Beste中鉴定了113,565和126,444蛋白。我们针对NCBI非冗余(nr)数据集执行了BLASTP注释所识别的蛋白质。综上所述,我们通过RNA-Seq提供了两个不同杏品种的转录组。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号