Genetic Factors Controlling the Proliferative Activity of Mouse Epidermal Melanocytes during the Healing of Skin Wounds

摘要

A cut was made on the middorsal skin of newborn mice of strains C57BL/10J, C57BL/10J-A/A, and C3H/He using fine iridectomy scissors. In the epidermis within 1 mm of the wound edge in C57BL/10J and C57BL/10J-A/A, the melanocyte population positive to the dopa reaction as well as the melanoblast-melanocyte population positive to the combined dopa-premelanin reaction increased dramatically until the 3rd day, then gradually decreased. In contrast, the melanocyte population of C3H/He did not increase after wounding, despite that the melanoblast-melanocyte population increased. Pigment-producing melanocytes in mitosis were frequently found in C57BL/10J and C57BL/10J-A/A, but not in C3H/He. The F(1), F(2), and backcross matings were performed to get some information about the genetic basis of the difference between C57BL/10J and C3H/He. In the F(1) generation the offspring from reciprocal crosses exhibited intermediate values in both populations on the 3rd day after wounding. The F(2) generation included the C3H/He type, F(1) type, and C57BL/10J type in a ratio of 1:2:1 in both populations. Moreover, both reciprocal backcrosses gave 1:1 ratios of parent type to F(1) type in both populations. These results indicate that the proliferative activity of mouse epidermal melanocytes during the healing of skin wounds are controlled by semidominant genes.