Leptin receptor expression on T lymphocytes modulates chronic intestinal inflammation in mice

摘要

>Background: Leptin regulates appetite through the long isoform of its receptor in the hypothalamus. Although leptin regulates immune responses, it is still unknown whether a direct effect of leptin on lymphocytes is required.>Aims: To clarify whether expression of leptin receptors on T lymphocytes modulates intestinal inflammation in mice.>Methods: The model of colitis induced by transfer of CD4⁺CD45RB^high (RB^high) cells into scid mice was used. Wild-type (WT) or leptin receptor deficient (db/db) RB^high cells were transferred into scid mice and development of colitis evaluated.>Results: Leptin receptors were expressed on both RB^high and RB^low cells. Intestinal lymphocytes of mice with colitis expressed high leptin levels compared with healthy controls whereas the opposite was true for serum leptin levels. Transfer of RB^high cells from db/db mice induced delayed disease compared with transfer of WT cells. A high rate of apoptosis in lamina propria lymphocytes and reduced cytokine production were observed early on in scid mice receiving db/db RB^high cells. These effects were not due to the high levels of glucocorticoids present in db/db mice as administration of corticosterone to WT mice failed to reproduce this phenomenon. High expression of peroxisome proliferator activated receptor γ was observed in the colon of recipients of db/db compared with WT cells. Freshly isolated db/db RB^high cells produced low levels of interferon γ. Despite delayed onset of colitis, as disease progressed differences between mice receiving WT or db/db cells were no longer apparent.>Conclusions: These results suggest that leptin affects the immune response, partly by acting on the long isoform of its receptor expressed on T lymphocytes.