Microdissection of the Ah01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome

摘要

BackgroundChromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding.