Comparison of nested-multiplex Taqman SYBR Green real-time PCR in diagnosis of amoebic liver abscess in a tertiary health care institute in India

摘要

Background & objectives:Amoebiasis is a common parasitic infection caused by Entamoeba histolytica and amoebic liver abscess (ALA) is the most common extraintestinal manifestation of amoebiasis. The aim of this study was to standardise real-time PCR assays (Taqman and SYBR Green) to detect E. histolytica from liver abscess pus and stool samples and compare its results with nested-multiplex PCR.