Enhanced resistance to fluoroquinolones in laboratory-grown mutants clinical isolates of Shigella due to synergism between efflux pump expression mutations in quinolone resistance determining region

摘要

Background & objectives:There is a worldwide emergence of fluoroquinolone resistance in Shigella species. To understand the molecular mechanisms associated with fluoroquinolone resistance, naturally occurring fluoroquinolone-resistant strains and laboratory-induced spontaneous mutants of Shigella spp. were used and the relative contributions of acrAB-tolC efflux pumps, gyrase and topoisomerase target gene mutations towards fluoroquinolone resistance were determined.