首页> 美国卫生研究院文献>Infection and Immunity >Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels.
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Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels.

机译:霍乱弧菌O1对绒毛和淋巴滤泡上皮的依从性:体外模型使用福尔马林处理过的人小肠其依从性与细胞相关血凝素水平相关。

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摘要

Formalin-fixed human small intestinal mucosa possessing villi and lymphoid follicle epithelium of Peyer's patches at the mucosal surface was used to test the adherence ability of clinically isolated strains of Vibrio cholerae O1. V. cholerae O1 grown on CFA agar for approximately 3 h at 37 degrees C had various levels of cellular hemagglutinins (HAs) and manifested adherence abilities that were roughly correlated with the cellular HA levels, irrespective of cellular HA types. V. cholerae O1 adhered better to epithelium over ileal lymphoid follicles than to epithelium of jejunal or ileal villi. Cells of different morphology which constituted lymphoid follicle epithelium were almost equal targets for adherence. In contrast, V. cholerae O1 grown on CFA agar for approximately 20 h at 37 degrees C in many cases had lower levels of cellular HAs and adherence abilities. Contrary to the above observations with cellular HAs and adherence, piliation of V. cholerae O1 was rather more extensive at approximately 20 h of incubation at 37 degrees C than at approximately 3 h of incubation at 37 degrees C. L-Fucose inhibited adherence to a varied extent depending on the cellular HA types, while D-mannose enhanced adherence in some strains. Heating of V. cholerae O1 diminished adherence ability. This adherence model system provides a tool by which various V. cholerae O1 strains can be preliminarily tested for adherence ability and site in human small intestine.
机译:用福尔马林固定的人小肠粘膜在粘膜表面具有绒毛和淋巴结的淋巴滤泡上皮来测试临床分离的霍乱弧菌菌株O1的粘附能力。在37°C下于CFA琼脂上生长约3小时的霍乱弧菌O1具有各种水平的细胞血凝素(HA),并且表现出与细胞HA水平大致相关的粘附能力,而与细胞HA类型无关。霍乱弧菌O1比回肠淋巴滤泡更能粘附在上皮上,而不是空肠或回肠绒毛上皮。构成淋巴滤泡上皮的不同形态的细胞几乎是相同的粘附靶标。相反,在许多情况下,霍乱弧菌O1在CFA琼脂上于37摄氏度下生长大约20小时,其细胞HA含量和粘附能力较低。与上述关于细胞HA和粘附的观察结果相反,霍乱弧菌O1在37摄氏度孵育约20小时的毛发比在37摄氏度孵育约3小时的毛发更为广泛。取决于细胞HA类型的不同程度,而D-甘露糖可增强某些菌株的粘附性。霍乱弧菌O1的加热降低了粘附能力。该粘附模型系统提供了一种工具,通过该工具可以预先测试各种霍乱弧菌O1菌株在人小肠中的粘附能力和部位。

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