Plaque developed in 10 occlusal fissures from unerupted third molars during implantation for 200 to 270 days in lower molars of dental students was studied. To characterize the predominant cultivable flora, 592 isolates (51 to 67 from each fissure) were subcultured from anaerobic roll tubes. Twenty-eight of the isolates were lost. Streptococci constituted 8 to 86% (median, 45%) of the isolates, Streptococcus mutans constituted 0 to 86% (median, 25%) and S. sanguis constituted 0 to 15% (median, 1%). A few isolates of "S. mitior" and "S. milleri" were found, but no S. salivarius. Staphylococci made up 0 to 23% (median, 9%). Gram-positive rods constituted 6 to 59% (median, 35%). Of these, 0 to 46% (median, 18%) were Actinomyces naeslundii and A. viscosus, but no anaerobic actinomyces were isolated. Arachnia and propionibacteria made up small proportions, lactobacilli were isolated from two fissures, constituting 10 and 29%, and eubacteria were isolated from one fissure (27%). Gram-negative cocci made up 0 to 46% (media, 4%). Only two isolates of gram-negative rods were found, both facultative anaerobes. Although 8 of the 10 fissures had large proportions of S. mutans, lactobacilli, or both, no caries was found even with microradiography. The large individual variation probably reflects differences in initial colonization from saliva and in growth conditions in each fissure.
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机译：研究了在牙科学生的下磨牙中植入200至270天期间，从未磨牙的第三磨牙中形成的10个咬合裂隙中的牙菌斑。为了表征主要的可培养菌群，从厌氧转管传代培养了592个分离株（每个裂隙有51至67个）。丢失了28株。链球菌占分离株的8％至86％（中位数，占45％），变形链球菌占0％至86％（中位数，占25％），血链球菌占0％至15％（中位数，占1％）。发现了一些“ S. mitior”和“ S. milleri”的分离株，但未发现唾液链球菌。葡萄球菌占0％至23％（中位数为9％）。革兰氏阳性棒占6％至59％（中位数为35％）。在这些中，有0至46％（中位数为18％）是内生放线菌和粘链曲霉，但没有分离出厌氧放线菌。蛛网膜炎和丙酸杆菌构成的比例很小，从两个裂缝中分离出乳酸杆菌，分别占10％和29％，从一个裂缝中分离出真细菌（27％）。革兰氏阴性球菌占0％至46％（媒体占4％）。仅发现两个革兰氏阴性菌的分离株，均为兼性厌氧菌。尽管10个裂缝中有8个具有较大比例的变形链球菌，乳杆菌或两者兼有，但即使使用X射线照相也未发现龋齿。较大的个体差异可能反映了每个裂缝中唾液的初始定植和生长条件的差异。
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