In vivo polyester immobilized sortase for tagless protein purification

摘要

BackgroundLaboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface of polyester (polyhydroxyalkanoate, PHA) beads in vivo in Escherichia coli by genetically fusing them to a polyester synthase gene (phaC). The insertion of a self-cleaving module, a modified sortase A (SrtA) from Staphylococcusaureus and its five amino acid recognition sequence between the synthase and the target protein led to a simple protein production and purification method.