Comparison of Germ Cell Gene Expressions in SpontaneousMonolayer versus Embryoid Body Differentiation of MouseEmbryonic Stem Cells toward Germ Cells

摘要

BackgroundGenetic and morphologic similarities between mouse embryonic stem cells (ESCs) and primordial germ cells (PGCs) make it difficult to distinguish differentiation of these two cell types in vitro. Using specific GC markers expressed in low level or even not expressed in ESCs- can help recognize differentiated cells in vitro. We attempted to differentiate the mouse ESCs into Gc-like cells spontaneously in monolayer and EB culture method.