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Haemocytometry by laser-beam optics: evaluation of the Hemac 630L.

机译:激光光束光学法:Hemac 630L的评估。

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摘要

Hemac is an automated blood counting system which is based on production of impulses from light scattered and diffracted by cells flowing past a laser beam. The pulses are processed electronically for cell counts and packed cell volume; haemoblobin is measured as cyanmethaemoglobin; absolute values are computed. On evaluation the precision was of the order of 2% for Hb and RBC, 2-8% for PVC, and 3% for WBC. When compared with other methods in the routine laboratory, PCV and absolute values gave divergent results, but PCV was found to correlate well with microhaematocrit corrected for trapped plasma. This suggests that Hemac may more closely reflect true values of PCV, MCV, and MCHC than other routine methods. Whether this "accuracy" is necessarily an advantage must be considered ietation of the blood count data, especially MCV, are discussed.
机译:Hemac是一种自动血液计数系统,该系统基于流过激光束的细胞散射和衍射的光产生的脉冲。对脉冲进行电子处理以获取细胞计数和填充细胞体积;血红蛋白测定为氰基高铁血红蛋白;计算绝对值。经评估,Hb和RBC的精度约为2%,PVC的精度为2-8%,WBC的精度为3%。与常规实验室中的其他方法相比,PCV和绝对值给出了不同的结果,但发现PCV与针对滞留血浆校正的微红细胞压积密切相关。这表明,相比其他常规方法,Hemac可能更接近地反映了PCV,MCV和MCHC的真实值。这种“准确性”是否一定是优势,必须考虑对血细胞计数数据,尤其是MCV的初始化。

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