The pivotal role of micro-environmental cells in a human blood–brain barrier in vitro model of cerebral ischemia: functional and transcriptomic analysis

摘要

Expression levels of tight junction proteins, ABC-transporters, VE-cadherin (CDH5), SLC2A1 (GLUT-1) and VEGFa in hCMEC/D3 cells cultivated in EBM-2 medium with 5% FCS for 5 days and incubated in EBM-2 medium with a reduced serum amount of 0.25% for additional 24 h from day 5 to day 6. After normalization to endogenous control b-actin and correction by individual primer efficacies, claudin-1 expression was set to 1000. Data are presented as mean ± SEM, n = 4 from four independent experiments