Ag-specific CD4 T cells promote innate immune responses in liver ischemia reperfusion injury

摘要

Ag-specific CD4 T cells exacerbate liver IRI. Cells from spleens and LNs of either OT II or WT mice were stained with fluorochrome-labeled Abs and subjected to FACS. WT or OT II T cells were adaptively transferred into congenic nude mice at 50 × 10 /mouse. FACS analysis of PBLs from these reconstituted nude mice was performed on day 6 post-reconstitution. Serum ALT measured after 90 m of ischemia and 6 h of reperfusion in OTII, CD8 KO, or WT mice, nude mice reconstituted with OT II or WT T cells, nude mice with liver injuries (Ctl), or nude mice reconstituted with naïve (n-OT II) or activated OT II (a-OT II) with or without treatment with MR1 (a-OT II/MR1). In vitro stimulation of OT II T cells with their cognate Ag, i.e., OVA peptide, activated these cells and FACS histograms show the levels of T cell activation markers. Liver histological images of different nude mouse groups after IRI (H/E staining). Suzuki scores. Liver inflammatory gene expression levels were measured by qRT-PCR, and Target gene/HPRT ratios were plotted.  = 5–6/group, *