hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in aNeonatal HIE Rat Model

摘要

In this study, we investigated how human umbilical cord mesenchymal stem cells exerted aneuroprotective effect via antiapoptotic mechanisms in a neonatal hypoxic-ischemicencephalopathy rat model. A total of 78 10-day old (P10) rats were used. After humanumbilical cord mesenchymal stem cells were collected from human umbilical cords andamplified in culture, they were administered to rat subjects 1 h after inducedhypoxic-ischemic encephalopathy treatment. The short-term (48 h) and long-term (28 day)outcomes were evaluated after human umbilical cord mesenchymal stem cells treatment usingneurobehavioral function assessment. Triphenyltetrazolium chloride monohydrate stainingwas performed at 48 h. Beclin-2 and caspase-3 levels were evaluated with Western blot andreal time polymerase chain reaction at 48 h. Human umbilical cord mesenchymal stem cellswere collected and administrated to hypoxic-ischemic encephalopathy pups byintracerebroventricular injection. Hypoxic-ischemic encephalopathy typically inducedsignificant delay in development and caused impairment in both cognitive and motorfunctions in rat subjects. Human umbilical cord mesenchymal stem cells were shown toameliorate hypoxic-ischemic encephalopathy-induced damage and improve both cognitive andmotor functions. Although hypoxic-ischemic encephalopathy induced significant expressionof caspase-3 and Beclin-2, human umbilical cord mesenchymal stem cells decreased theexpression of both of them. Human umbilical cord mesenchymal stem cells may serve as apotential treatment to ameliorate brain injury in hypoxic-ischemic encephalopathypatients.