Kinesin-6 KIF20B is required for efficient cytokinetic furrowing and timely abscission in human cells

摘要

Cytokinesis requires the cooperation of many cytoskeletal and membrane regulators. Most of the major players required for cytokinesis are known, but the temporal regulation and adaptations for different cell types are less understood. KIF20B (previously called MPHOSPH1 or MPP1) is a member of the Kinesin-6 family, which also includes the better-known members KIF23/MKLP1 and KIF20A/MKLP2. Previously, we showed that mouse Kif20b is involved in cerebral cortex growth and midbody organization of neural stem cells. Here, using siRNA-mediated knockdown of KIF20B in a human cell line and fixed and live imaging, we show that KIF20B has a cell-autonomous role in cytokinesis. KIF20B depletion affects the speed of both furrow ingression and abscission. It localizes to microtubules of the central spindle and midbody throughout cytokinesis, at sites distinct from the other Kinesin-6 family members. KIF20B is not required for midbody assembly, but may accelerate or coordinate midbody maturation. In particular, KIF20B appears to regulate late steps of maturation including anillin dispersal, ESCRT-III recruitment, and the formation of microtubule constriction sites.