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A Highlights from MBoC Selection: Plasma membrane domains enriched in cortical endoplasmic reticulum function as membrane protein trafficking hubs

机译:MBoC选择的亮点:富含皮质内质网的质膜结构域起着膜蛋白运输中心的作用

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摘要

In mammalian cells, the cortical endoplasmic reticulum (cER) is a network of tubules and cisterns that lie in close apposition to the plasma membrane (PM). We provide evidence that PM domains enriched in underlying cER function as trafficking hubs for insertion and removal of PM proteins in HEK 293 cells. By simultaneously visualizing cER and various transmembrane protein cargoes with total internal reflectance fluorescence microscopy, we demonstrate that the majority of exocytotic delivery events for a recycled membrane protein or for a membrane protein being delivered to the PM for the first time occur at regions enriched in cER. Likewise, we observed recurring clathrin clusters and functional endocytosis of PM proteins preferentially at the cER-enriched regions. Thus the cER network serves to organize the molecular machinery for both insertion and removal of cell surface proteins, highlighting a novel role for these unique cellular microdomains in membrane trafficking.
机译:在哺乳动物细胞中,皮质内质网(cER)是与质膜(PM)紧密对置的小管和储水池网络。我们提供的证据表明,富集在基础cER中的PM域充当了HEK 293细胞中PM蛋白插入和去除的运输枢纽。通过使用全内反射荧光显微镜同时可视化cER和各种跨膜蛋白货物,我们证明了回收的膜蛋白或膜蛋白首次被递送至PM的大多数胞外递送事件发生在富含cER的区域。同样,我们观察到优先在cER富集区域反复出现网格蛋白簇和PM蛋白的功能性内吞作用。因此,cER网络可用于组织分子机制以插入和去除细胞表面蛋白,从而突出了这些独特的细胞微域在膜运输中的新作用。

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