βarrestin-1 regulates DNA repair by acting as an E3-ubiquitin ligase adaptor for 53BP1

摘要

βarr1 binds to 53BP1. Co-immunoprecipitation (co-IP) experiments were performed from cell lysates derived from MEF cells endogenously expressing βarr1 and 53BP1. Clarified lysates were incubated with either, anti-βarr1 (K-16) antibody, anti-53BP1 antibody, or IgG and the resulting products resolved by SDS-PAGE and probed by western blot analysis using antibodies directed against 53BP1 and βarr1. 53BP1 and βarr1 expression in cytosol and nucleus