Properties and pharmacological modification of ATP-sensitive K+ channels in cat tracheal myocytes

摘要

class="enumerated" style="list-style-type:decimal">The effects of levcromakalim and nucleoside diphosphates (NDPs) on both membrane currents and unitary currents in cat trachea myocytes were investigated by use of patch-clamp techniques.In conventional whole-cell configuration, levcromakalim produced a concentration-dependent K⁺ current which was suppressed by additional application of 5 μM glibenclamide at −70 mV. When 3 mM ATP was added in the pipette solution, the peak amplitude of the levcromakalim-induced current was much smaller than that in the absence of ATP.When 3 mM uridine 5′-diphosphate (UDP) was included in the pipette solution, much higher concentrations of glibenclamide (⩾50 μM) were required to suppress the 100 μM levcromakalim-induced membrane current in comparison with those in the absence of UDP.In the cell-attached patches, levcromakalim activated a 40 pS K⁺ channel which was inhibited by additional application of glibenclamide in symmetrical 140 mM K⁺ conditions.UDP (⩾0.1 mM) was capable of reactivating the channel in inside-out patches in which the glibenclamide-sensitive K⁺ channel had run down, in the presence of levcromakalim. The K⁺ channel reactivated by UDP was suppressed by additional application of either intracellular 3 mM ATP or 100 μM glibenclamide.These results demonstrate that smooth muscle cells in the cat trachea possess ATP-sensitive 40 pS K⁺ channels which are blocked by glibenclamide (i.e. KATP) and can be activated by levcromakalim and that intracellular UDP causes a significant shift of the glibenclamide-sensitivity of these channels.