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A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology.

机译:一种估计前列腺素15-羟基脱氢酶活性的快速方法及其在药理学中的应用。

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摘要

A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.
机译:描述了一种新的前列腺素15-羟基脱氢酶(PGDH)的双同位素检测方法。 2该测定方法简单,快速,精确,对PGDH完全特异,并且没有伪影。获得的结果与常规测定法很好地相关。 3该方法用途广泛,可用于评估无细胞系统和灌注器官中的酶活性,非常适用于PGDH抑制剂的测定。

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