A Study of Methods for Producing Cell-free Tumour Antigen from BP8 Mouse Ascites Tumour

摘要

One million lymph node cells (LNC) from C57 B1 mice immunized with BP8 cells plus Freund's complete adjuvant (FCA) protected C3H mice against fatal challenge with BP8 injected i.p. 20 hours later. The LNC of C57 B1 mice immunized with the supernatant from BP8 cells treated with lysolecithin (1·5 μg/10⁶ cells) gave equally good protection. BP8 cells after treatment with lysolecithin equalled untreated BP8 cells in antigenic efficiency. Cell-free preparations made from BP8 cells with 3 molar KCl were of doubtful antigenic efficiency. The LNC from C57 B1 mice immunized with C3H liver and spleen tissue plus FCA gave no protection and LNC from non-immunized C57 B1 mice did not protect C3H mice against BP8 tumour.Centrifugation of the supernatants at 105,000 g indicated that the antigenic properties of the lysolecithin supernatant might be due to subcellular particles which were not found in the 3 molar KCl preparations.