Circaea mollis Siebold Zucc. Alleviates postmenopausal osteoporosis in a mouse model via the BMP-2/4/Runx2 pathway

摘要

Effect of EECM on osteoblast differentiation of MC3T3-E1 cells and osteoclast differentiation of Raw264.7 cells. Cell viability after 48 h of EECM treatment in MC3T3-E1 osteoblasts. ALP activity in MC3T3-E1 after cells were differentiated for 7 days in differentiation medium with EECM. TRAP activity in RAW264.7 cells after culturing for 7 days with RANKL (50 ng/mL). ARS staining in MC3T3-E1 cells and ( ) ARS-stained cells. Western blot analysis and ( ) real-time PCR analysis of osteoblastic markers in MC3T3E1 cells. *  p p