Cathepsin K regulates localization and secretion of Tartrate-Resistant Acid Phosphatase (TRAP) in TRAP-overexpressing MDA-MB-231 breast cancer cells

摘要

TRAP isoform prevalence in MDA-MB-231 cells. One representative Western blot of TRAP isoform expression and processing is shown in mock control and TRAP3 cell lysate. Membranes were stained with anti-total TRAP antibody and anti-β-Actin antibody. Quantification of TRAP isoform 5a (uncleaved TRAP, 35 kDa) and 5b (cleaved TRAP, ~ 23 kDa/N-terminal and ~ 15 kDa/C-terminal) expression in mock control and TRAP3 cell lysates from several Western blots with normalization to β-Actin (42 kDa) and statistically compared by ANOVA test (Fisher’s LSD test, independent experiments mock ctrl:  = 10, TRAP3  = 9, bar graphs represent means and Standard error, B). **