Crystallization of a protein using dehydration without a precipitant

摘要

Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 Å and belong to space group P41212 (or P43212), with unit-cell parameters a = b = 79.18, c = 37.34 Å.