Crystallization and preliminary crystallographic analysis of endo-13-β-glucanase from Arthrobacter sp.

摘要

Endo-1,3-β-glucanases hydrolyze internal 1,3-β-glucosyl linkages. The endo-1,3-β-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P41, with unit-cell parameters a = 71.31, c = 60.07 Å, and contained one molecule per asymmetric unit. The Matthews coefficient (V M) and the solvent content were 2.35 ų Da⁻¹ and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 Å at SPring-8 using a MAR CCD area detector and gave a data set with an overall R merge of 5.4% and a completeness of 99.4%.