Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type 2Fe–2S ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102

摘要

The electron-transfer complex of BphA3, a Rieske-type [2Fe–2S] ferredoxin, and BphA4, a NADH-dependent ferredoxin reductase, was crystallized using the sitting-drop vapour-diffusion method under anaerobic conditions. The obtained crystals were analyzed by SDS–PAGE, which showed that they contained both BphA3 and BphA4. The crystals belong to space group P21, with unit-cell parameters a = 60.60, b = 173.72, c = 60.98 Å, β = 115.8°, and diffracted to a resolution of 1.9 Å.