Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor σ28 in complex with the σ28-binding region of its antisigma factor FlgM

摘要

The sigma 28 kDa (σ²⁸) factor is a transcription factor specific for the expression of bacterial flagellar and chemotaxis genes. Its antisigma factor, FlgM, binds σ²⁸ factor and inhibits its activity as a transcription factor. In this study, crystals of the complex between Escherichia coli σ²⁸ and the C-terminal σ²⁸-binding region of FlgM were obtained. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 106.7 (2), c = 51.74 (3) Å, containing one complex in the crystallographic asymmetric unit. An X-ray intensity data set was collected to a resolution of 2.7 Å.