Crystallization and preliminary X-ray studies of ferredoxin-NADP+ oxidoreductase encoded by Bacillus subtilis yumC

摘要

Ferredoxin-NADP⁺ oxidoreductase encoded by Bacillus subtilis yumC has been purified and successfully crystallized in complex with NADP⁺ in two forms. Diffraction data from crystals of these two forms were collected at resolutions of 1.8 and 1.9 Å. The former belonged to space group P21212, with unit-cell parameters a = 63.90, b = 135.72, c = 39.19 Å, and the latter to space group C2, with unit-cell parameters a = 207.47, b = 64.85, c = 61.12 Å, β = 105.82°. The initial structure was determined by the molecular-replacement method using a thioredoxin reductase-like protein as a search model.