Crystallization of the novel S-adenosyl-l-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

摘要

Recombinant protein from Streptomyces rishiriensis (CouO), an S-­adenosyl-l-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P21, with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.