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New crystallization plate for biological macromolecule, and method for crystallizing biological macromolecule using the same, and method for collecting directly X-ray diffraction data from biological macromolecule crystal produced thereby
New crystallization plate for biological macromolecule, and method for crystallizing biological macromolecule using the same, and method for collecting directly X-ray diffraction data from biological macromolecule crystal produced thereby
The present invention relates to a novel biopolymer crystallization plate, biopolymer crystallization method using the same, and thus the direct collection method of the X- ray diffraction data from the crystals produced by biopolymer. Biopolymer crystallization plate according to the present invention is a biopolymer mixture solution holder (2) by including an area of crystal formation area (3) parts are in perforated center of the top, when crystallizing biopolymers large and well-arranged shape biopolymer crystals the can get. In addition, after the solidification of the crystallization solution can be mounted directly by jikgeo biopolymer crystallization plate in X- ray diffraction without using the determined sampled loop directly X- ray diffraction data collected from crystals biopolymer containing a biopolymer crystal minimizes the damage to the bio-polymer crystals, by using a cryogenic freezing for injecting liquid nitrogen X- ray it can be prevented from degradation (deterioration) of the biopolymer crystals in the diffraction data collection process, up to the biopolymer crystallization plate 120 ° rotation by the X- ray diffraction data can be collected until it biopolymer X- ray diffraction data collected in a large-scale screening is possible and sufficient availability X- ray to determine the three-dimensional structure of the biopolymer diffraction data (full data set ) it may be the acquisition. Therefore, it is possible to develop a large-scale equipment to confirm the essential biomolecular research on three-dimensional structure, such as structure-based drug discovery. ;
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