Designing Anti-inflammatoryDrugs from Parasitic Worms:A Synthetic Small Molecule Analogue of the Acanthocheilonemaviteae Product ES-62 Prevents Development of Collagen-InducedArthritis

摘要

In spite of increasing evidence that parasitic worms may protect humans from developing allergic and autoimmune diseases and the continuing identification of defined helminth-derived immunomodulatory molecules, to date no new anti-inflammatory drugs have been developed from these organisms. We have approached this matter in a novel manner by synthesizing a library of drug-like small molecules based upon phosphorylcholine, the active moiety of the anti-inflammatory Acanthocheilonema viteae product, ES-62, which as an immunogenic protein is unsuitable for use as a drug. Following preliminary in vitro screening for inhibitory effects on relevant macrophage cytokine responses, a sulfone-containing phosphorylcholine analogue (>11a) was selected for testing in an in vivo model of inflammation, collagen-induced arthritis (CIA). Testing revealed that >11a was as effective as ES-62 in protecting DBA/1 mice from developing CIA and mirrored its mechanism of action in downregulating the TLR/IL-1R transducer, MyD88. >11a is thus a novel prototype for anti-inflammatorydrug development.