Designof Sealable Custom-Shaped Cell Mimicries Basedon Self-Assembled Monolayers on CYTOP Polymer

摘要

In bottom-up synthetic biology, one of the major methodological challenges is to provide reaction spaces that mimic biological systems with regard to topology and surface functionality. Of particular interest are cell- or organelle-shaped membrane compartments, as many protein functions unfold at lipid interfaces. However, shaping artificial cell systems using materials with non-intrusive physicochemical properties, while maintaining flexible lipid interfaces relevant to the reconstituted protein systems, is not straightforward. Herein, we develop micropatterned chambers from CYTOP, a less commonly used polymer with good chemical resistance and a refractive index matching that of water. By forming a self-assembled lipid monolayer on the polymer surface, we dramatically increased the biocompatibility of CYTOP-fabricated systems. The phospholipid interface provides an excellent passivation layer to prevent protein adhesion to the hydrophobic surface, and we succeeded in cell-free protein synthesis inside the chambers. Importantly, the chambers could be sealed after loading by a lipid monolayer, providing a novel platformto study encapsulated systems. We successfully reconstituted pole-to-poleoscillations of the Escherichia coli MinDE system, which responds dramatically to compartment geometry.Furthermore, we present a simplified fabrication of our artificialcell compartments via replica molding, making it a readily accessibletechnique for standard cleanroom facilities.