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共1675条结果
  • 1.

    【2hr】The potential of the newly isolated thermotolerant Kluyveromyces marxianus for high-temperature ethanol production using sweet sorghum juice

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    机译 新分离的耐热马克斯克鲁维酵母使用甜高粱汁生产高温乙醇的潜力
    摘要：In this work, the newly isolated thermotolerant Kluyveromyces marxianus DBKKUY-103 exhibited a high ethanol fermentation efficiency at high temperatures using sweet sorghum juice (SSJ). The highest ethanol concentrations and productivities achieved under the optimum conditions using thermotolerant K. marxianus DBKKUY-103 were 85.16 g/l and 1.42 g/l.h at 37 °C and 83.46 g/l and 1.39 g/l.h at 40 °C, respectively. The expression levels of genes during ethanol fermentation at 40 °C were evaluated and the results found that the transcriptional levels of the RAD10, RAD14, RAD33, RAD50, ATPH, ATP4, ATP16, and ATP20 genes were up-regulated compared with those at 30 °C, suggesting that the high growth and high ethanol production efficiencies of K. marxianus DBKKUY-103 during high-temperature ethanol production associated with the genes involved in DNA repair and ATP production.
  • 2.

    【2hr】Comparison of response surface methodology and artificial neural network to enhance the release of reducing sugars from non-edible seed cake by autoclave assisted HCl hydrolysis

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    机译 响应面法与人工神经网络通过高压灭菌辅助HCl水解增强非食用种子饼中还原糖释放的比较
    摘要：In the current investigation, statistical approaches were adopted to hydrolyse non-edible seed cake (NESC) of Pongamia and optimize the hydrolysis process by response surface methodology (RSM). Through the RSM approach, the optimized conditions were found to be 1.17%v/v of HCl concentration at 54.12 min for hydrolysis. Under optimized conditions, the release of reducing sugars was found to be 53.03 g/L. The RSM data were used to train the artificial neural network (ANN) and the predictive ability of both models was compared by calculating various statistical parameters. A three-layered ANN model consisting of 2:12:1 topology was developed; the response of the ANN model indicates that it is precise when compared with the RSM model. The fit of the models was expressed with the regression coefficient R2, which was found to be 0.975 and 0.888, respectively, for the ANN and RSM models. This further demonstrated that the performance of ANN was better than that of RSM.
  • 3.

    【2hr】Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

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    机译 萝卜（Raphanus sativus，L。）的优良毛状根作为抗氧化剂和类黄酮的来源
    摘要：An efficient protocol for hairy root induction in radish was established by optimizing several parameters that affect the efficiency of Agrobacterium rhizogenes-mediated transformations. Explants wounded using sterile hypodermic needle, infected with Agrobacterium suspension (0.6 OD600) for 10 min and co-cultivated in 1/2 MS medium containing acetosyringone (100 µM) for 2 days displayed maximum percentage of hairy root induction using MTCC 2364 (77.6%) and MTCC 532 (67.6%). On further experiments with MTCC 2364 initiated hairy roots, maximum biomass accumulation (fresh weight = 9.50 g; dry weight = 1.48 g) was achieved in liquid 1/2 MS medium supplemented with 87.6 mM sucrose after 40 days of culture. Transgenic state of hairy roots of MTCC 2364 was confirmed by polymerase chain reaction using rolB- and rolC-specific primers. The MTCC 2364-induced hairy roots produced higher amount of phenolic (33.0 mg g−1), flavonoid (48.0 mg g−1), and quercetin (114.8 mg g−1) content compared to auxin-induced roots of non-transformed radish. Furthermore, the results of ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl assay confirmed that the antioxidant activity of MTCC 2364 root extracts was improved when compared to auxin-induced roots of non-transformed radish. The present study offers a new insight in radish for production of phenolics and flavonoids (quercetin) using A. rhizogenes-mediated hairy root induction.
  • 4.

    【2hr】Evidence of antidiabetic activity of Spirulina fusiformis against streptozotocin-induced diabetic Wistar albino rats

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    机译 螺旋藻对链脲佐菌素诱导的糖尿病Wistar白化病大鼠抗糖尿病活性的证据
    摘要：The aim of our study is to investigate the protective effect of Spirulina fusiformis against streptozotocin-induced diabetes in Wistar albino rats. Rats were divided into five groups: group I was normal control, group II was diabetic control (50 mg/kg b.w. of streptozotocin, i.p.), group III was Spirulina fusiformis (400 mg/kg b.w., p.o.) treated diabetic rats; group IV was Glibenclamide (0. 6 mg/kg b.w., p.o.) treated diabetic rats and group V was treated with Spirulina fusiformis (400 mg/kg b.w., p.o.) alone. There was significant elevation in the levels of blood glucose, serum lipid profile and serum renal markers (total protein, urea, creatinine and uric acid) in the diabetic rats. Also, diabetic rats showed significantly (P < 0.05) reduced antioxidant status (reduced levels of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione; increased levels of TBARS), impaired oral glucose tolerance and elevated HbA1C. Spirulina fusiformis was able to normalize the above mentioned parameters. Significant histopathological changes were found in the pancreas, liver and kidney sections of the diabetic control group while treatment with Spirulina fusiformis was able to minimize the extent of tissue damage. Current study shows that Spirulina fusiformis possesses significant antidiabetic and antihyperlipidemic effects in streptozotocin-induced diabetic rats by effectively reducing the rise in blood glucose levels and lipid profile.
  • 5.

    【2hr】Anaerobic digestion of sulphate-rich post-tanning wastewater at different COD/sulphate and F/M ratios

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    机译 不同COD /硫酸盐和F / M比的富硫酸盐制革后废水的厌氧消化
    摘要：Anaerobic digestion of post-tanning wastewater was performed in batch anaerobic digester to evaluate the effect of COD/sulphate ratio [0.62, 0.69, and 1.20 (w/w) %] and F/M ratio [0.2, 0.3, 0.5, 0.7, 0.9, 1.1, 1.3, and 1.5 (w/w) %)] on the removal efficiency of COD. The F/M ratio of 0.3 was found to be the optimum ratio for the removal of COD by 53, 57, and 65%, respectively at COD/sulphate ratio of 0.62, 0.69, and 1.20. The maximum sulphate removal was observed at F/M ratio of 0.2 and the removal efficiency was 48, 50, and 58% at COD/sulphate ratio of 0.62, 0.69, and 1.20, respectively. The removal efficiency of COD and sulphate was increased with increase in COD/sulphate ratio from 0.62 to 1.20 and decreased with increase in F/M ratio from 0.2 to 1.5 in anaerobic digestion of post-tanning wastewater. The maximum concentration of sulphide formation was 784 mg/L at COD/sulphate ratio of 0.62 in anaerobic digestion process and the process was inhibited at this sulphide concentration. The microbial activity in the sludge was evaluated through live and dead cell assay using fluorescent microscopy. The maximum amount of dead microbes was observed in the anaerobic digester, which was operated at COD/sulphate ratio of 0.62 than other studied ratio.
  • 6.

    【2hr】Glyphosate induced toxicity to chickpea plants and stress alleviation by herbicide tolerant phosphate solubilizing Burkholderia cepacia PSBB1 carrying multifarious plant growth promoting activities

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    机译 草甘膦诱导对鹰嘴豆植物的毒性，并通过耐除草剂的磷酸盐增溶了洋葱伯克霍尔德氏菌PSBB1，从而减轻了压力，并具有多种促进植物生长的活性
    摘要：In this study, strain PSBB1 isolated from Vicia faba rhizosphere was identified as Burkholderia cepacia, by 16S rDNA sequence analysis and characterized. Strain PSBB1 tolerated glyphosate up to 3200 μg ml−1 and produced IAA (81.6 μg ml−1), ACC deaminase (69.3 mg−1 protein h−1), SA (39.3 μg ml−1) and 2,3-DHBA (26.6 μg ml−1), solubilized insoluble P (50.8 μg ml−1) and secreted 29.4 μg ml−1 exopolysaccharides, which decreased with increasing concentrations of glyphosate. Cell damage following glyphosate application was visible under SEM and CLSM. The phytotoxicity of glyphosate on chickpea was variable but significant. B. cepacia mitigated toxicity and enhanced the size, dry matter, symbiosis, seed attributes and nutritional contents of chickpea. Further, B. cepacia strain PSBB1 declined the levels of CAT, POD, APX and GPX and MDA contents at 4332 μg kg−1 soil glyphosate. Proline also increased under glyphosate stress but declined in B. cepacia inoculated plants. The ability to tolerate higher concentration of glyphosate, the capacity to secrete plant growth regulators even under herbicide stress and potential to reduce the level of proline and antioxidant enzymes makes B. cepacia as an interesting choice for enhancing chickpea production in soils contaminated even with herbicides.
  • 7.

    【2hr】Draft genome sequence of Paenibacillus strain LK1, a phytohormone producing bacterium

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    机译 产生植物激素的细菌Paenibacillus LK1菌株的基因组序列草案
    摘要：A high-quality draft genome sequence of a multi-antibiotic resistant strain, Paenibacillus strain LK1 isolated from the rhizosphere of Auricularia auricular is described. Strain LK1 produced several types of phytohormones, which included Indole-3-acetic acid (IAA) and Zeatin. Phylogenetic analysis of the 16S rRNA gene of strain LK1 showed a close relationship to P. taichungensis and P. pabuli (99% gene sequence similarity). The genome size of strain LK1 was estimated to be 7.16 Mb that consisted of 82 contigs contained in 49 scaffolds. The G + C content of the genome was 45.63% and it encoded 6499 genes, 115 pseudo genes, 8 rRNAs, 33 tRNAs, and 4 ncRNAs, based on the Prokaryotic Genome Annotation Pipeline (PGAP). Further analysis of the genome of strain LK1 identified 16 genes encoding monooxygenase and six genes encoding cytochrome P450, which may be potentially involved in zeatin biosynthesis. The draft whole genome sequence and annotation of strain LK1 has been deposited at DDBJ/EMBL/GenBank under the accession number ().
  • 8.

    【2hr】Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

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    机译 杜仲芽孢杆菌分离的病原菌具有SSF抑制和木质纤维素分解酶的完整基因组序列157
    摘要：Bacillus velezensis 157 was isolated from the bark of Eucommia ulmoides, and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, B. velezensis 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes. Soybean meal appeared to be the most efficient substrate for the single fermentation of B. velezensis 157. Highest yield of pectinase (19.15 ± 2.66 U g−1), cellulase (46.69 ± 1.19 U g−1) and amylase (2097.18 ± 15.28 U g−1) was achieved on untreated soybean meal. Highest yield of xylanase (22.35 ± 2.24 U g−1) was obtained on untreated wheat bran. Here, we report the complete genome sequence of the B. velezensis 157, composed of a circular 4,013,317 bp chromosome with 3789 coding genes and a G + C content of 46.41%, one circular 8439 bp plasmid and a G + C content of 40.32%. The genome contained a total of 8 candidate gene clusters (bacillaene, difficidin, macrolactin, butirosin, bacillibactin, bacilysin, fengycin and surfactin), and dedicates over 15.8% of the whole genome to synthesize secondary metabolite biosynthesis. In addition, the genes encoding enzymes involved in degradation of cellulose, xylan, lignin, starch, mannan, galactoside and arabinan were found in the B. velezensis 157 genome. Thus, the study of B. velezensis 157 broadened that B. velezensis can not only be used as biocontrol agents, but also has potentially a wide range of applications in lignocellulosic biomass conversion.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1125-2) contains supplementary material, which is available to authorized users.
  • 9.

    【2hr】Identification of a monopartite begomovirus associated with yellow vein mosaic of Mentha longifolia in Saudi Arabia

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    机译 沙特阿拉伯与薄荷叶黄脉花叶病相关的单倍体夜蛾病毒的鉴定
    摘要：Mentha is a very important crop grown and used extensively for many purposes in the Kingdom of Saudi Arabia. Begomoviruses are whitefly-transmitted viruses causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. During farmers’ field survey, yellow vein mosaic disease was observed in Mentha longifolia plants growing near tomato fields in Saudi Arabia. The causative agent was identified in 11 out of 19 samples using begomovirus-specific primers and the association of begomovirus with yellow vein mosaic disease in M. longifolia was confirmed. The full-length viral genome and betasatellite were amplified, cloned, and sequenced bidirectionally. The full DNA-A genome was found to have 2785 nucleotides with 1365 bp-associated betasatellite molecule. An attempt was made to amplify DNA-B, but none of the samples produced any positive amplicon of expected size which indicated the presence of monopartite begomovirus. The sequence identity matrix and phylogenetic analysis, based on full genome showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) and in phylogenetic analysis it formed a closed cluster with Tomato leaf curl virus infecting tomato and Corchorus crop in Saudi Arabia. The sequence analysis results of betasatellites showed the highest identity (98.9%) with Tomato yellow leaf curl betasatellites infecting tomato and phylogenetic analysis using betasatellites formed a close cluster with Tomato yellow leaf curl betasatellites infecting tomato and Corchorus crops, which has already been reported to cause yellow vein mosaic and leaf curl disease in many cultivated and weed crops growing in Saudi Arabia. The identified begomovirus associated with yellow vein mosaic disease in mentha could be a mutated strain of TYLCV and tentatively designated as TYLCV-Mentha isolate. Based on published data and latest information, this is the first report of identification of Tomato yellow leaf curl virus associated with yellow vein mosaic disease of M. longifolia from Saudi Arabia.
  • 10.

    【2hr】Preliminary molecular detection of the somatic embryogenesis receptor-like kinase (VpSERK) and knotted-like homeobox (VpKNOX1) genes during in vitro morphogenesis of Vanilla planifolia Jacks

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    机译 香草平叶杰克逊体外形态发生过程中体细胞胚发生受体样激酶（VpSERK）和打结的同源盒（VpKNOX1）基因的初步分子检测。
    摘要：This work aimed to evaluate the embryogenic competence of different tissues from different stages (friable callus, bud-regenerating callus, and whole buds) of Vanilla planifolia, through the molecular detection of the somatic embryogenesis receptor-like kinase (VpSERK) and knotted-like homeobox (VpKNOX1) genes. RNA was extracted with Trizol®, cDNA was obtained, and the studied transcripts were amplified. Using non-specific primers, VpSERK and VpSTM gene expression was detected in the three stages evaluated. This study might contribute to providing an explanation for the recalcitrance of this Vanilla species to somatic embryogenesis.
  • 11.

    【2hr】Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes

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    机译 产黄青霉菌株HKF2的基因组序列草案，该真菌具有产生益生元合成酶的潜力
    摘要：In this study, we have characterized a novel set of extracellular enzymes produced by Penicillium chrysogenum strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with Penicillium rubens, Penicillium chrysogenum followed by Penicillium expansum and Penicillium roqueforti. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1132-3) contains supplementary material, which is available to authorized users.
  • 12.

    【2hr】Understanding the molecular basis of differential grain protein accumulation in wheat (Triticum aestivum L.) through expression profiling of transcription factors related to seed nutrients storage

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    机译 通过与种子营养物质存储相关的转录因子表达谱分析，了解小麦（Triticum aestivum L.）小麦籽粒蛋白质积累差异的分子基础
    摘要：Increasing nutritional value of cereals is one of the important research and breeding objectives to overcome malnutrition in developing countries. The synthesis of grain seed proteins during grain filling is controlled by several mechanisms including transcriptional and posttranscriptional modifications. In the current investigation, transcript abundance analysis of three allelic variants of seed storage protein activator (Spa A, Spa B and Spa D) and NAM-B1 affecting seed nutrient concentration was carried out in two genotypes (UP 2672 and HS 540) of bread wheat differing in grain protein content. Expression profiling of transcription factor genes was performed using quantitative real time PCR (qRT-PCR). Positive correlation and significant p value > 0.05 was observed among the fold expression in developing stages of both the genotypes. Maximum expression of Spa genes was observed at S3 stage and maximum fold expression was observed for Spa B gene in case of UP 2672, the genotype with high protein content. The transcript profiling of NAM-B1 gene revealed threefold higher expression in UP 2672 than that of HS 490 at S4 stage. The findings revealed the role of transcriptional regulation in differential grain protein accumulation through varied expression and existence of their allelic variants in wheat genotypes.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1114-5) contains supplementary material, which is available to authorized users.
  • 13.

    【2hr】Flavonoid-rich extract of Chromolaena odorata modulate circulating GLP-1 in Wistar rats: computational evaluation of TGR5 involvement

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    机译 广色草富含类黄酮的提取物调节Wistar大鼠循环GLP-1：TGR5参与的计算评估
    摘要：Chromolaena odorata is a major bio-resource in folkloric treatment of diabetes. In the present study, its anti-diabetic component and underlying mechanism were investigated. A library containing 140 phytocompounds previously characterized from C. odorata was generated and docked (Autodock Vina) into homology models of dipeptidyl peptidase (DPP)-4, Takeda-G-protein-receptor-5 (TGR5), glucagon-like peptide 1 (GLP1) receptor, renal sodium dependent glucose transporter (SGLUT)-1/2 and nucleotide-binding oligomerization domain (NOD) proteins 1&2. GLP-1 gene (RT-PCR) modulation and its release (EIA) by C. odorata were confirmed in vivo. From the docking result above, TGR5 was identified as a major target for two key C. odorata flavonoids (5,7-dihydroxy-6-4-dimethoxyflavanone and homoesperetin-7-rutinoside); sodium taurocholate and C. odorata powder included into the diet of the animals both raised the intestinal GLP-1 expression versus control (p < 0.05); When treated with flavonoid-rich extract of C. odorata (CoF) or malvidin, circulating GLP-1 increased by 130.7% in malvidin-treated subjects (0 vs. 45 min). CoF treatment also resulted in 128.5 and 275% increase for 10 and 30 mg/kg b.w., respectively. Conclusions: The results of this study support that C. odorata flavonoids may modulate the expression of GLP-1 and its release via TGR5. This finding may underscore its anti-diabetic potency.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-018-1138-x) contains supplementary material, which is available to authorized users.
  • 14.

    【2hr】Molecular structure and catalytic mechanism of fungal family G acidophilic xylanases

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    机译 真菌家族G嗜酸木聚糖酶的分子结构和催化机理
    摘要：Industrial applications of xylanases have made this enzyme an important subject of applied research work. Function of this particular enzyme is to degrade or hydrolyze the plentiful polysaccharide xylan, an important component of hemicellulose. It mainly cleaves the backbone of xylan that is made up of a number of xylose residues connected with β-1,4-glycosidic linkages. Fungi with mycelia are regarded as the best producer of xylanases. These varied xylanases not only differ in their sizes and shapes but also differ in their physicochemical properties. Depending on the optimum pH in which they work best, they have been classified into (1) acidophilic xylanases active at low pH or acidic pH range, (2) alkaliphilic xylanases that are active at high or alkaline pH range and (3) neutral xylanases having pH optima in the neutral range between pH 5 and 7. Other researchers have classified the xylanases also on the basis of their structural properties, kinetic parameters, etc. This review discusses the molecular structures of some acidophilic xylanases and the molecular basis of low pH optima observed for their activities. It also discusses their unique catalytic mechanism and actual role of the catalytic residues found in them. Apart from these, the review also discusses different applications of these acidophilic xylanases in different industries. The article concludes with brief suggestions about how these acidophilic xylanases can be created employing the techniques of genetic engineering and concepts of synthetic evolution, using the traits of the known acidophilic xylanases discussed in the review.
  • 15.

    【2hr】Synthesis of gold nanomaterials and their cancer-related biomedical applications: an update

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    机译 金纳米材料的合成及其与癌症有关的生物医学应用：最新动态
    摘要：Recently, the advances in the synthesis of new types of nanomaterials have created several opportunities in drug delivery and targeted therapy applications. Among the various nanostructures, gold nanostructures with controllable physical and chemical properties have received attention for various biomedical uses, including sensing of biomolecules, in vitro and in vivo bioimaging (as advanced contrast agents for photothermal and bioimaging techniques), photothermolysis of cancer cells, and targeted drug delivery. The attractive properties of gold nanomaterials, particularly, anti-angiogenic properties, are highly useful in a variety of cancers studies. In addition, they can bind many proteins and drugs and can be actively targeted to cancer cells over-expressing cell surface receptors and they are biocompatible in nature with a high atomic number, which directs to greater absorption of kilovoltage X-rays and provides greater contrast than standard agents. In this review, we have summarized the synthesis, structure and functionalization of gold nanostructures, and their biomedical applications with special reference to cancer studies.
  • 16.

    【2hr】Molecular marker of high glycyrrhizic acid content based on the SNPs of liquorice IPT gene

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    机译 基于甘草IPT基因SNPs的高甘草酸含量分子标记
    摘要：Previous studies found that cytokinins (CTKs) are able to significantly promote glycyrrhizic acid (GA) accumulation. This study analyzed the correlation between the contents of GA and CTKs in 98 one-year-old liquorice plants by the combination of high-performance liquid chromatography and enzyme-linked immunosorbent assay. The full length of the liquorice IPT gene was cloned and the Single-nucleotide polymorphisms (SNPs) of the IPT gene in the samples were obtained. The molecular marker of high GA content was found by performing correlation analysis on the SNPs of the IPT gene. Results indicated that the contents of GA and CTKs were positively correlated. The full-length sequence of the IPT gene was 1002 bps, and five SNPs were found in the 98 liquorice samples. One of five SNPs was 348 bps, exhibiting missense mutation and the rest displaying synonymous mutations. In the five SNPs, 348 bps was primarily associated SNP, whereas 821 and 848 bps were auxiliary associated SNP. The findings of this study provided insight into the molecular mechanism of GA genetic variation, serving as basis for improving the GA content in cultivated liquorice and the good varieties breeding.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1085-6) contains supplementary material, which is available to authorized users.
  • 17.

    【2hr】High yield production of folates from soybean callus cultures in response to elicitors

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    机译 从大豆愈伤组织培养物中高产叶酸，以应对激发子
    摘要：Soybean (Indian variety, JS 335) callus growth and its folate content was sustained on Murashige and Skoog (MS) medium supplemented with optimized levels of auxins and cytokinins. Callus cultures exhibited moderate production of total folate. Callus growth was stimulated with highest biomass content of 21.3 g/L DW (24 days culture) on medium supplemented with 1.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L Kinetin (Kn). Total folate production was enhanced by an increase in indole-3-acetic acid (IAA) from 0.5 to 1.0 mg/L and decreased thereafter up to 1.5 mg/L. 2,4-D at 1.5 mg/L repressed the callus growth and also folate production. A concentration of 1.0 mg/L IAA and 0.2 mg/L Kn elicited folate production to a maximum total folate content of 323.82 µg/100 g DW. The results indicate that optimized medium for callus suspension cultures conceivably are applied for scale-up studies in bioreactors.
  • 18.

    【2hr】Design of peptide-based epitope vaccine and further binding site scrutiny led to groundswell in drug discovery against Lassa virus

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    机译 基于肽的表位疫苗的设计和进一步的结合位点审查导致针对拉萨病毒的药物发现大放异彩
    摘要：Lassa virus (LASV) is responsible for an acute viral hemorrhagic fever known as Lassa fever. Sequence analyses of LASV proteome identified the most immunogenic protein that led to predict both T-cell and B-cell epitopes and further target and binding site depiction could allow novel drug findings for drug discovery field against this virus. To induce both humoral and cell-mediated immunity peptide sequence SSNLYKGVY, conserved region 41–49 amino acids were found as the most potential B-cell and T-cell epitopes, respectively. The peptide sequence might intermingle with 17 HLA-I and 16 HLA-II molecules, also cover 49.15–96.82% population coverage within the common people of different countries where Lassa virus is endemic. To ensure the binding affinity to both HLA-I and HLA-II molecules were employed in docking simulation with suggested epitope sequence. Further the predicted 3D structure of the most immunogenic protein was analyzed to reveal out the binding site for the drug design against Lassa Virus. Herein, sequence analyses of proteome identified the most immunogenic protein that led to predict both T-cell and B-cell epitopes and further target and binding site depiction could allow novel drug findings for drug discovery field against this virus.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1106-5) contains supplementary material, which is available to authorized users.
  • 19.

    【2hr】Identification and molecular characterization of Dof transcription factor gene family preferentially expressed in developing spikes of Eleusine coracana L.

    包量

    机译 Dof转录因子基因家族的鉴定和分子特征优先在发展中国家的斑潜蝇的穗中表达。
    摘要：We report 48 putative DNA binding with one finger (Dof) TF genes from genome and transcriptome data of finger millet (Eleusine coracana L.; FM), involved in plant developmental process. To characterize seed-specific Dof genes, transcript profiles of 32 EcDof identified from transcriptome data of developing spikes of FM genotypes were further analyzed in different tissues (root, stem, and leaf) and developmental stages of spikes (S1, S2, S3, and S4) in two FM genotypes [GE1437 (low protein genotype; LPG) and GE3885 (high protein genotype; HPG)]. More than 50% of identified EcDof genes showed expression during seed development processes. Among these, seven genes (EcDof 3, EcDof 5, EcDof 15, EcDof 18, EcDof 22, EcDof 23, and EcDof 31) expressed maximally at specific stages of seed development. Fourteen EcDof genes showed that differential transcript accumulation in vegetative tissue as well as in developing spikes suggests involvement during seed filling and also throughout the plant development. In addition, three EcDof genes (EcDof 9, EcDof 25, and EcDof 28) expressed preferentially at root and stem tissue. The 3D structural prediction of EcDof proteins showed variability in structural attributes. Molecular docking results showed strong binding affinity for seed-specific EcDof–EcO2 with α-prolamine promoters. The identified and characterized EcDof genes will help to dissect the roles of FM seed-specific Dof genes.Electronic supplementary materialThe online version of this article (10.1007/s13205-017-1068-z) contains supplementary material, which is available to authorized users.
  • 20.

    【2hr】Carica papaya (Papaya) latex: a new paradigm to combat against dengue and filariasis vectors Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

    包量

    机译 番木瓜（木瓜）乳胶：对抗登革热和丝虫病媒介的新范式埃及伊蚊和库蚊（Qulex quinquefasciatus）（双翅目：葫芦科）
    摘要：This study manifests the larvicidal efficacy of Carica papaya latex extract and silver nanoparticles (CPAgNPs) synthesized using latex, against developing immature juveniles of Aedes aegypti and Culex quinquefasciatus. Briefly, the latex was collected and fractioned with different solvents such as chloroform, methanol and aqueously. The obtained crude extracts were subjected to larvicidal activity in the dose-dependent method. After 24 h, the mortality rate was calculated and statistically analyzed. From the results, it was demonstrated that the chloroform extract displayed prominent activity in IInd and IIIrd instar larvae of A. aegypti and C. quinquefasciatus with better LC50 values followed by methanol and aqueous extract. Subsequently, we profiled the qualitative analysis of a chloroform extract through biochemical tests; Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Moreover, we authenticated the major secondary metabolites and activated larvicidal compound present in the extract. Further, we synthesized CPAgNPs using aqueous latex extract and challenged with IInd and IIIrd instar larvae of A. aegypti and C. quinquefasciatus. Noticeably, the synthesized nanoproducts were showed 100% mortality in a 24-h treatment with significant LC50 values. Hence, this study has opened up new vistas in the field of parasitological research to develop Carica papaya latex as a new stratagem in the insect vector management program.
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