大肠癌中microRNA-93的表达及其与临床病理因素的关系

摘要

Objective To investigate the expression of microRNA- 93 in colorectal carcinoma and its relation to clinico-pathological features of the cancer. Methods The expression of microRNA- 93 stem- loop real- time RT- PCR in cancer tissue and matched pericancerous tissue specimens from 42 patients with colorectal carcinoma. The relationship between microR-NA- 93 expression and clinicopathologic features of colorectal carcinoma was analyzed. Results The expression level of mi-croRNA- 93 was significantly higher in colorectal carcinoma than that in pericancerous tissues (P<0.05). The expression of mi-croRNA- 93 was positively correlated with the lymph node metastasis and depth of tumor invasion (P<0.05 or 0.01), not with age, sex, tumor size, distant metastasis and celldifferentiation(P>0.05). The sensitivity and specificity of microRNA- 93 expression for evaluation of lymph node metastasis were 0.867 and 0.556 respectively;those for evaluation of tumor invasion depth were 0.800 and 0.833, respectively. Conclusion The over- expression of microRNA- 93 may be associated with development and progres-sion of colorectal carcinoma. The expression of microRNA- 93 might be used as a adjuvant parameter for predicting the lymph node metastasis and depth of tumor invasion in colorectal carcinoma.%目的探讨大肠癌中microRNA-93的表达及其与临床病理因素的关系。方法取42例大肠癌及癌旁正常黏膜组织标本，采用茎环逆转录实时荧光定量PCR方法检测大肠癌及癌旁正常黏膜组织中microRNA-93的表达水平，并比较分析其在不同临床病理因素间的表达差异。结果（1）microRNA-93在大肠癌组织中的表达水平显著高于癌旁正常黏膜组织，差异有统计学意义（P＜0.01）；（2）microRNA-93的表达与大肠癌淋巴结转移和浸润深度有关（P＜0.05或0.01）；而与患者的年龄、性别、肿瘤大小、远处转移、分化程度无关（均P＞0.05）；（3）microRNA-93评价大肠癌淋巴结转移的敏感性和特异性分别为0.867和0.556，评价大肠癌浸润深度的敏感性和特异性为0.800和0.833。结论 microRNA-93表达上调可能与大肠癌的发生、发展有关，microRNA-93可作为评价大肠癌淋巴结转移和浸润深度的辅助指标。