目的：观测酪酸梭菌是否对阿司匹林导致的胃溃疡具有预防效应，并对其机制进行初步探讨。方法取30只健康雄性ICR小鼠，随机分成3组，每组各10只，即模型组、酪酸梭菌组、奥美拉唑组。酪酸梭菌组以6×108cfu/只的菌量，模型组和奥美拉唑组均予以相同体积的无菌培养基，灌胃4d后再饥饿处理48h，然后以250mg/kg剂量的阿司匹林灌胃造模，奥美拉唑组小鼠于造模前30min，腹腔注射奥美拉唑（13mg/kg）。造模4h后，取胃组织分别测定SOD、CAT活力、MDA含量；进行常规HE染色观察胃黏膜组织结构变化，PAS染色判断黏液量的变化，免疫组化法测定Bcl-2、Bax表达水平。结果模型组的SOD、CAT活性明显降低，MDA含量显著增加，而酪酸梭菌预处理后逆转了该趋势（P＜0.01），与奥美拉唑组比较无统计学差异。HE染色观察，酪酸梭菌能减轻胃黏膜的病理损伤，与奥美拉唑组无统计学差异。PAS结果提示酪酸梭菌能够增加黏液量。酪酸梭菌组的Bcl-2表达较模型组显著增多，同时Bax表达显著减少。结论酪酸梭菌预处理通过抗氧化应激、增加胃黏液量、上调Bcl-2/Bax表达比例，减轻阿司匹林造成的胃溃疡损伤。%Objective To investigate the effect of Clostridium butyricum (C. butyricum) in prevention of gastric ulcer (GU) induced by aspirin in mice. Methods Thirty ICR mice were randomly divided into model group, bacteria group andomeprazole group (n=10/group). After starvation for 48h, mice were given oral y with 250mg/kg aspirin to establish GU model.Mice in bacteria group were oral y pretreated with C. butyricum 6 ×108cfu for 4d before starvation, while mice in model group and omeprazole groupwere given the samevolume of sterile culture medium.Mice in omeprazole group were intraperitoneal y injected with 13mg/kg omeprazole 30 min priorto aspirin treatment. Modeling for 4 h, gastric tissue was to harvest for measuring the activity of SOD and CAT, the content ofMDA, Bcl- 2 and Bax, HE staining and PAS staining were performed. Results In model group, the activity of SOD, CAT were significantly reduced while the content of MDA was significantly increased. The activity of SOD,CAT were in-creased while the content of MDA was reduced in bacteria group (P<0.01), there was no significant difference between bacteria and omeprazole group. The Bcl- 2 expression increased and Bax expression reduced in bacteria group. PAS stainning showed that the mucus secretion was increased in bacteria group and HE staining showed that the mucosa injury was attenuated in bacte-ria gorup. Conclusion C. butyricum can effectively protect the gastric mucosa against damages caused by aspirin in mice.
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