非小细胞肺癌患者外周血中miRNA-4286表达的临床意义

摘要

目的 通过检测非小细胞肺癌(NSCLC)患者外周血中miRNA-4286的表达水平,探讨其在NSCLC诊断中的临床意义.方法 收集31例NSCLC患者作为观察组,20例肺部良性肿瘤患者作为良性对照组,21例健康体检者作为阴性对照组;采用实时荧光定量PCR技术检测各组外周血中miRN A-4286的表达水平;采用循环阈值(CT)法(△CT=CT内参基因-CT目标基因)进行miRNA-4286定量分析.结果 外周血miRNA-4286的表达水平:观察组术前为△CT=-15.423±3.631、术后1周为△CT=-1 1.179±1.296,良性对照组为△CT=-9.459±1.019,阴性对照组为△CT=-9.459±1.019;观察组手术前后比较差异有统计学意义(t=-13.598,P＜0.05);观察组术前与良性对照组、阴性对照组比较差异均有统计学意义(t=-16.032、-14.021,均P＜0.05).结论 miRNA-4286在NSCLC患者外周血中表达水平明显低于良性肿瘤和健康人群,且术后1周较术前明显升高,提示外周血miRNA-4286低表达可能与NSCLC发生、发展相关.%Objective To investigate the expression of miRNA-4286 in peripheral blood of patients with non-small cell lung cancer (NSCLC) and its clinical significance.Methods Thirty one patients with NSCLC (NSCLC group),20 patients with benign lung tumor (benign control group) and 21 healthy subjects (normal control group) were enrolled in the study.The expression levels of miRNA-4286 in peripheral blood were detected by quantitative real-time PCR (qRT-PCR),and presented as △ cycle threshold (△ CT) (△ CT=CTreference-CTtarget gene).Results The expression level (△ CT) in peripheral blood of miRNA-4286 was-15.423 ± 3.631 in NSCLC group before operation and-11.179 ± 1.296 one week after operation;-9.014 ± 0.995 in benign control group and-9.459 ± 1.019 in normal control group.There were a significant differences in NSCLC group before and after treatment(t=-13.598,P＜0.05),between NSCLC group before treatment and benign control group (t=-16.032,P＜9.95),between NSCLC group before treatment and normal control group (t=-14.021,P ＜0.05).Conclusion The expression level of miRNA-4286 in peripheral blood is significantly lower in patients with NSCLC than that of benign tumors and healthy subjects,and it is increased after operation in NSCLC patients,which suggests that down-regulation of miRNA-4286 may be associated with the development of NSCLC.