大鼠脑出血后IL-1β和CD163/HO-1信号通路表达及其与脑水肿的相关性

摘要

Objective To investigate the relationship between the CD163/HO-1 pathway, IL-1βand edema in perihematoma tissue of exper-imental cerebral hemorrhagic rats. Methods The rat model of experimental ICH was established by injection of arterial blood into the caudate nu-cleus, the expression of HO-1, CD163 and IL-1β were detected by using immunohistochemistry at different time. Brain water content was measured. Results The expression of HO-1,CD163 and IL-1β appeared at 24 hour after ICH, peaked at 72 hour, then decreased gradually, there was a posi-tive correlation between HO-1,CD163 and IL-1β(P<0.05), and the brain water content(P<0.05). Conclusions The CD163/HO-1 pathway played an important role in inflammatory reactions in perihematoma tissue around experimental ICH rats, and was correlated with encephaledema of the ICH rats.%目的：探讨脑出血后CD163/HO-1信号通路与IL-1β表达的动态变化，分析其与脑水肿的关系。方法 SD大鼠90只，随机分为正常对照组、假手术组、模型组、分别在造模后24小时、72小时、7天处死动物，免疫组化法检测HO-1、CD163、IL-1β的表达，并测定脑组织含水量。结果 ICH大鼠模型组24h后灶周可见IL-1β、HO-1、CD163阳性细胞增多，IL-1β阳性细胞数脑出血组与假手术组相比增多（P＜0.01），假手术组IL-1β阳性细胞数多于正常对照组（P＜0.01）。 HO-1阳性细胞数脑出血组、假手术组均高于正常对照组（P＜0.01），脑出血组高于假手术组，在72小时明显增多（P＜0.01），CD163假手术组在72小时高于正常对照组（P＜0.01），各时间点脑出血组明显高于假手术组及正常对照组（P＜0.01）。脑组织含水量72小时最高，与HO-1、CD163正相关（P＜0.05）。结论 CD163、HO-1与 ICH后炎性因子 IL-1β的表达相关，CD163/HO-1信号通路与ICH后脑水肿有关。