萝卜硫素增加HL-60和人原代白血病细胞对化疗药敏感性实验研究

摘要

Objective: To investigate the effect of sulforaphane (SF) on chemotherapy sensitivity of leukemia cells. Methods: Semi-solid agar colony culture assay and MMT method were used to observe the effect of different con-centrations of SF on HL-60 cell proliferation and homoharringtonine+ ara-c chemotherapy on cell growth. PHA-LCM two-stage culture method was used to detect the effect of SF and homoharringtonine+ara-c chemotherapy on human primary leukemia cells. Results: With a certain range of concentrations, SF inhibited the proliferation of HL-60 cells and the growth of human primary leukemia cells in a dose-dependent manner (P<0.05,P<0.01). SF enhanced homoharringtonine+ara-c chemotherapy sensitivity of HL-60 cells (P<0.05) and human primary leukemia cells. Conclusion: SF can inhibit the proliferation of leukemia cells with a certain range of concentrations and en-hance chemotherapy sensitivity of these cells.%　　目的：探讨萝卜硫素（SF）对白血病细胞化疗药物敏感性的作用。方法：琼脂半固体集落培养法和MTT法检测不同浓度的萝卜硫素对HL-60细胞增殖的影响及协同化疗药对细胞生长的抑制作用；PHA-LCM液相原半固体二步培养SF协同化疗药抑制人原代白血病细胞生长。结果：琼脂半固体集落培养法和MTT法证实在一定浓度范围内SF对HL-60细胞增殖抑制（P<0.05，P<0.01），呈剂量依赖性，SF能够增强HL-60细胞对化疗药物高三尖杉酯碱（Hom）和阿糖胞苷（Ara-c）敏感性（P<0.05）；PHA-LCM液相原半固体二步培养法证实SF在一定浓度范围内对人原代白血病细胞有抑制作用（P<0.05，P<0.01），呈剂量依赖性，并增加人原代白血病细胞对化疗药Hom和Ara-c敏感性。结论：萝卜硫素在一定浓度下有效抑制白血病细胞增殖，并能增强化疗药物的敏感性。