桑叶总黄酮对糖尿病高脂血症大鼠血糖、血脂和胸主动脉p22phox mRNA表达的影响

摘要

目的：探讨桑叶总黄酮（FML）对糖尿病高脂血症大鼠血糖、血脂和胸主动脉p22phox mRNA表达的影响。方法60只雄性SD大鼠随机分成空白组、模型组、FML低、中、高剂量组和西药组，每组10只，观察FML对大鼠血糖、血脂、胸主动脉p22phox mRNA表达的影响。结果与空白组比较，模型组大鼠血糖、血脂显著升高（P<0.01），胸主动脉p22phox mRNA表达明显上调（P<0.01）。与模型组比较，FML中、高剂量组及西药组大鼠血糖、血脂降低（P<0.05或P<0.01），FML中、高剂量组及西药组大鼠胸主动脉p22phox mRNA表达明显下调（P<0.05或P<0.01）。结论 FML显著降低糖尿病高脂血症大鼠血糖、血脂水平，其机制可能与FML下调大鼠胸主动脉p22phox mRNA表达，进而抑制NADPH氧化酶活化有关。%Objective To investigate the influence of total flavonoids from mulberry leaves (FML) on blood glu-cose, blood lipid and the expression of p22phox mRNA in thoracic aorta in rats with diabetes and hyperlipidemia. Methods Diabetes and hyperlipidemia rats were used in this study. Sixty SD male rats were randomly into blank group, model group, treatment groups (high, middle, low dose of FML and western medicine group), 10 in each group. The effect of FML on blood glucose, blood lipid and the expression of p22phox mRNA in thoracic aorta was observed. Results Blood glucose, blood lipid and the expression of p22phox mRNA in thoracic aorta in rats in model group were higher than those in blank group(P<0.01). Compared with model group, rats in middle and high dose of FML groups and western medicine group had lower blood glucose, blood lipid and decreased expression of p22phox mRNA in thoracic aorta (P<0.05 or P<0.01). Conclusion FML can inhibit levels of blood glucose and blood lipids in diabetes and hyperlipidemia rats. This effect may be due to decreasing the expression of p22phox mRNA in thoracic aorta and then the activation of NADPH oxidase.