吐鲁番部分疫区牛环形泰勒虫病流行病学调查

摘要

[Objective]This study was designed to conduct the epidemiological investigation of infection of Theileria annulata for cattle in some epidemic areas of Turpan.[Method] A number of suspected Theileria annulata disease cases (n =126) in Turpan were diagnosed by molecular method:Polymerase chain reaction and then the result was compared with that of blood smear method of 126 samples.And cattle were divided into groups by age and farm respectively and analyzed by statistical methods.[Result] The infection rate of Theileria annulata for 126 suspected cases was 51.6％ (65/126),while that of blood smear method was 47.6％ (60/126).The difference between different farms or ages were both significant (P ＜ 0.05).[Conclusion] Turpan is the epidemic region of Theileria annulata.The only way to cure the disease is to diagnose it early by molecular method,eliminate intermediary ticks,and take feasible comprehensive precautionary measures to keep it in control.%[目的]调查了解吐鲁番部分疫区牛环形泰勒虫病的流行情况.[方法]应用PCR方法和病原学血液涂片法对吐鲁番地区部分疑似牛环形泰勒虫病病例(n=126)进行了分子诊断,并用病原学方法对阳性头份的结果进行了分析、验证.[结果]该疫区126头份疑似病例的PCR方法检测的阳性结果为51.6％ (65/126),病原学检查-血涂片染色镜检的阳性率为47.6％ (60/126),而不同牧场、不同年龄间的感染率差异显著(P＜0.05).[结论]吐鲁番地区是牛环形泰勒虫病的流行区域,用分子生物学方法进行早期诊断,患牛才有治愈的可能,并且消灭媒介蜱,制定切实可行的综合防控措施.