To investigate chorionic villous vasculogenesis(maturation)and development of the vasculosyncytial membrane(margination)using CD34immunohistochemistry.C ase -control study.Microscopic analysis of first trimester chori-onic villi.Twelve patients with anembryonic pregnancies,12with embryonic death,and 12with t erminated normal pregnancies.Quantitative analysis of chorionic villi blinded to group and gestational age using CD34immunohisto-chemistry.Vascular parameters(mean functional vascular area,vessels with a lumen,and heman giogenetic cords,peripherally or centrally located).Terminated normalpregnancies show significantly more vessels per chorionic villus(maturation)(mean ±SEM)in comparison with embryonic deaths and anembryonic pr egnancies(5.3±0.3vs.1.4±0.2and 0.7±0.1),located mainly peripherally(margination)(3.0±0.2vs.0.9±0.2and 0.2±0.0).Anembryonic pregnancies show sign ifi-cantly more centrally located cords in comparison with embryonic deaths and termination of pregnancies(3.3±0.2vs.2.7±0.2and 1.5±0.1).A defective chori-onic villous vascularization,demo nstrating inadequate vasculogenesis and abnormal develo pment of the vascu-losyncytial membrane,is seen in pre gnancies complicated by embryonic death and is even more pr onounced in anembryonic pregnancies.Initiation of placental vasculo-genesis is a basic feature in all types of pregnancy and is subsequently modulated directly or indirectly by embryonic signaling.
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