Objective:To explore impact of Huazhuo Jiedu Huoxue Tongluo Method ( turbid resolving, toxin clearing, blood activating and collateral dredging ) on learning and memorizing ability and morphology of hippocampal CA1 region of mice with cerebral ischemia reperfusion injury. Methods: Fifty Kunming mice were randomized to model group, high dose Chinese medicine group, low dose Chinese medicine group, Nimodipine group, sham operation group. Mice in the groups had external carotid artery separated and ligated, except sham operation group whose artery were separated without ligation. After the models were established, the groups were given respective treatments, except sham operation group and model group which received saline. After treatment, behavior test was performed, morphology of hippocampal CA1 region was observed. Results: Compared with model group, mice in the Chinese medicine groups had better learning ability, and improved morphology of nerve cell. Conclusion: Huazhuo Jiedu Huoxue Tongluo method has protective effect for mice with cerebral ischemia reperfusion injury.%目的:观察应用化浊解毒活血通络法对脑缺血再灌注损伤小鼠学习记忆能力及海马CA1区神经细胞形态学的影响.方法:将50只昆明小鼠随机分为模型组、中药高剂量组、中药低剂量组、尼莫地平组、假手术组.前4组采用双侧颈总动脉结扎法合并尾端放血制造脑缺血再灌注模型,假手术组只分离颈总动脉不结扎.手术造模成功后假手术组、模型组灌服生理盐水,尼莫地平组灌服尼莫地平液,中药高剂量组、中药低剂量组分别给予高、低剂量的化浊解毒活血通络中药灌服.在治疗后进行行为学测试,并通过光镜观察小鼠海马CA1区的病理形态学改变.结果:与模型组相比,化浊解毒活血通络法能提高脑缺血再灌注损伤小鼠的学习成绩,改善小鼠海马CA1区的神经细胞形态学表现.结论:化浊解毒活血通络法对小鼠的脑缺血再灌注损伤有保护作用.
展开▼
