马齿苋水提取物的平喘作用研究

摘要

Objective:To evaluate the effects of WEPO on the anti-asthma and on tension of tracheal smooth muscle.Methods:Asth-matic model was induced by ultrasonic atomization inhalation of histamine phosphate-acetylcholine chloride in guinea pig.The guinea pigs whose incubation period of asthma was less than 120s were selected and divided randomly they into 5 groups which were 1.25 g/kg, 2.5 g/kg,5.0 g/kg of WEPO group,Ami 20 mg/kg group and control group.To evaluate anti-asthma the latent period induced asthma, the numbers of tracheal smooth muscle,the height of liquid in the capillary was recorded,and the difference of height of liquid between before and after treated by WEPO(Δh) and △h were computed.Results:The latent period induced asthma in low, medium and high-dose group were (108.2 ±19.1)s,(211.3 ±45.2)s and (226.9 ±41.5)s Respectively and these were lower than (59.5 ±10.8)s control group(P<0.05 or P<0.01).In WEPO medium and high-dose group the rate of asphyxia of animals were 40%and 30%re-spectively,and these were significantly lower than in control group( P<0.05).In low, medium and high dose group,Δh were ( -5.02 ±1.03),(-8.78 ±2.04),(-10.32 ±2.24) respectively and these were significantly lower than(-0.5 ±0.21) in control group(P<0.05 or P<0.01).In low, medium and high -dose group △h were (2.24 ±1.01),(2.96 ±1.42),(3.21 ±1.49),these were significantly lower than ( 0.71 ±0.45 ) in control group ( P<0.05 or P<0.01 ) .Conclusion:WEPO has significant anti-asthmatic effect and the effects are related with relaxation of tracheal smooth muscle and improve ventilation.%目的：观察马齿苋水提取物（ Water Extract of Portulaca Oleracea L，WEPO）平喘作用及对气管平滑肌张力的影响。方法：采用超声雾化磷酸组胺－氯化乙酰胆碱诱发豚鼠喘息模型，选取引喘潜伏期＜120 s豚鼠，随机分为WEPO 1．25 g/kg、2．5 g/kg、5．0 g/kg（生药）剂量组，阳性对照组（氨茶碱20 mg/kg）和空白对照组。以引喘潜伏期及因呼吸困难、窒息发生抽搐跌倒动物比率为指标，评价WEPO的平喘作用；采用豚鼠离体完整气管毛细管法，以毛细管液面高度的变化值（Δh）和△h为指标，观察WEPO对气管平滑肌张力的影响。结果：WEPO低、中、高3个剂量组豚鼠引喘潜伏期分别为（108．2±19．1） s，（211．3±45．2）s和（226．9±41．5）s，均明显长于空白对照组（59．5±10．8）s（P＜0．05或P＜0．01），WEPO中、高剂量组发生抽搐跌倒动物比率分别为40％和30％，显著低于空白对照组100％（P＜0．05）。 WEPO低、中、高剂量组Δh值分别为（－5．02±1．03），（－8．78±2．04），（－10．32±2．24），与空白对照组（－0．5±0．21）比较差异有统计学意义（P＜0．05或P＜0．01）；WEPO低、中、高剂量组△h值分别为（2．24±1．01），（2．96±1．42），（3．21±1．49）高于空白对照组（0．71±0．45）。结论：WEPO对豚鼠喘息模型有显著的平喘作用，其作用与松弛气管平滑肌，改善通气量有关。