目的：实验探讨附麻平喘方对哮喘模型大鼠的影响并探讨其作用机制。方法：选用SPF级清洁雄性大鼠60只，采用注射卵蛋白（OVA）与氢氧化铝致敏、OVA雾化吸入激发来复制哮喘动物模型，造模后将其随机分为5组：模型组（等容量蒸馏水）、附麻防喘片组（377.5 mg/kg）、附麻平喘方高剂量组（49.68 g/kg）、附麻平喘方中剂量组（24.84 g/kg）、附麻平喘方低剂量组（12.42 g/kg），每组10只。并于致敏2周后开始灌胃给药8周。末次给药后，采用ELISA法检测血浆ACTH，血清CORT、IL-4、IFN-γ及IgE的含量；取肺脏，一侧做HE染色，另一侧采用免疫组化法检测肺组织内β！ AR的含量。结果：附麻平喘方能有效缓解模型大鼠哮喘的症状，减轻肺组织病变程度，同时显著升高血清CORT，血浆ACTH，肺组织β！ AR的水平。结论：附麻平喘方可改善哮喘模型大鼠的症状，其机制可能与改善HPA轴及免疫功能有关。%Objective:To examine the effect of the Fuma Pingchuan Prescription for Asthma in Experimental Rats and to study its mech-anism.Methods:Sixty Clean male rats of SPF.Except the blank control group,all the rats were injected with ovalbumin(OVA)and alu-minum hydroxide sensitization,OVA inhalation excited to replicate animal models of asthma.The experimental rats were divided into five groups:the model group(such as volume of distilled water),positive compare group(377.5 mg/kg),Fuma Pingchuan Prescription high dose group(49.68 g/kg),middle dose group(24.84 g/kg)and low dose group(12.42 g/kg).Each group consists of ten rats.The rats started to receive intragastric administration 2 weeks after sensitization,1 dose each day in 8 weeks.After the last administration detected by ELISA in plasma ACTH,serum CORT,IL-4、IFN-γand IgE levels.Staining the tissue in one side of the lung with HE and detec-ting β-AR content.In the other side by immunohistochemistry.Results:Fuma Pingchuan Prescription can effectively alleviate asthma, reduce asthma symptoms and improve lung tissue morphology,while significantly elevated serum CORT,plasma ACTH,lung tissue β-AR levels.Conclusion:Fuma Pingchuan Prescription can improve asthma symptoms of model rats,which may be related to the improved functions of HPA axis and immune.
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