GC 法测定羚黄宝儿丸中麝香酮、冰片、丁香酚的含量

摘要

Objective:To determine the contents of Muscon,Borneo and Eugenol in Linghuangbaoer Pellets by GC method.Meth-ods:The GC system consisted of DB-WAX(30.0 m ×0.25 mm ×0.25 μm)capillary column.Programmed temperature was set from 120 ℃ to 210 ℃ and the detector temperature was 250 ℃.The detector was FID using high pure helium as carrier gas with the flow rate of 1.2 mL/min.Results:Borneol,Eugenol and Muscon were separated with a good linearity in the sample size ranges of 0.125 ~2 mg/mL for Borneol,0.05 ~0.8 mg/mL for Eugenol,and 0.005 ~0.08 mg/mL for Muscon.The average recoveries for Borneol,Eugenol and Muscon was 105.51% (RSD =1.44%),95.79% (RSD =0.29%),99.94% (RSD =0.66%),respec-tively.Conclusion:The GC method is simple,feasible and reproducible.It can be used for the quality control of Linghuangbaoer Pellets.%目的：建立 GC 法同时测定羚黄宝儿丸中麝香酮、冰片、丁香酚3种成分的含量。方法：采用直接进样 GC 法，色谱柱为 DB －WAX 毛细管柱（30.0 m ×0.25 mm ×0.25μm），FID 检测器，载气为氮气，程序升温，柱流量为1.2 mL／min。结果：3种成分在均呈现良好的线性关系，精密度、重复性 RSD 均＜3％，加样回收率在95％～106％。结论：直接进样 GC 法方法简便、灵敏、准确、重现性好，适用于羚黄宝儿丸中麝香酮、冰片、丁香酚的含量测定。