附子品种的抗病性及根表真菌多样性研究

摘要

Objective:To study the relationship between the disease resistance of Radix Aconiti Lateralis Praeparata and the diversity of fungi system under the guidance of systematic theory of Chinese herbal medicine.Methods:High-throughput technology was used to build the rhizoplane fungus library of different types of Radix Aconiti Lateralis Praeparata,and then the relationship between the diversity index and the anti-disease capacity was analyzed.Results:There were differences in the dominant strains and the diversity of fungi community for the rhizoplane soils between the Xiao-Hua Radix Aconiti Lateralis Praeparata and the Gua-Ye Radix Aconiti Lateralis Praeparata,the healthy and pathogenic Radix Aconiti Lateralis Praeparata;The root system of Radix Aconiti Lateralis Praeparata affect the structure of soil fungi.Atheli arolfsii,Mucor racemosus were the pathogenic fungi,while the Mortierella,Penicillium are antagonistic fungi to root rot pathogens.Conclusion:The results provide theoretical guidance for good quality management of Radix Aconiti Lateralis Praeparata agriculture,and enrich the theory and analysis methods for the system science of Chinese herbal medicine.%目的:在系统中药思想指导下,研究附子品种的抗病能力及与外界真菌系统多样性的关系.方法:使用高通量技术构建附子品种的根表真菌文库,分析其多样性指数与附子抗病品质形成的关系.结果:小花叶与瓜叶子型附子之间,以及健康与病害附子之间,其根表土壤的优势真菌种类、群落多样性均存在差异,附子根系影响了土壤真菌的结构组成,阿太菌、总状毛霉等是附子根腐病的致病菌群,而被孢霉属、青霉菌属等又组成病害菌的拮抗菌群.结论:本结果为附子农业的良好品质管理提供理论指导,也丰富了附子品种品质形成的系统学理论与分析方法.