基于氧化应激调控的芪参颗粒干预心力衰竭大鼠的机制研究

摘要

Objective:To study the effect and mechanisms of Qishen granules ( QSG ) on heart failure rats based on oxidative stress .Methods:Heart failure rat model was established by ligation of left coronary artery ( LAD) .Rats were randomly divided in-to four groups including sham group , model group , QSG group and Fosinopril group .The pathological changes of heart were as-sessed by Hematoxylin-Eosin ( HE) and Masson′s staining .Real-Time PCR and Western blot were respectively used to detect the mRNA and protein levels of p47phox and RAC1, the key molecules in oxidative stress in each group .Results:HE and Masson′s staining showed that the myocardial cells of rats in model group were disorderly arranged , and a large number of inflammatory cells were infiltrated in the interstitium .The collagen was excessively accumulated and the fibrous tissue was proliferated .On the con-trast, the myocardial cells of rats in QSG and sham group were arranged in an order way and the collagen deposition was hardly ob -served.Real-Time PCR (RT-PCR) and Western blotting results showed that mRNA and protein levels of p 47phox and RAC1 were increased in the model group , and QSG could significantly inhibit the expression of them .Conclusion:QSG may improve the heart function of heart failure rats induced by left anterior descending artery ligation , and its mechanisms is probably related to the inhi-bition of oxidative stress .%目的:探讨芪参颗粒干预心力衰竭大鼠的药效研究,并基于氧化应激探讨其作用机制.方法:制备左冠脉结扎诱导的心力衰竭大鼠模型,随机分为假手术组、模型组、芪参颗粒组、福辛普利组.通过苏木精-伊红染色(HE)法、Masson染色分别观察各组大鼠心肌病理变化,并采用实时荧光定量PCR(RT-PCR)和蛋白印迹(Western blotting)法分别检测大鼠心肌中氧化应激关键分子p47 phox、RAC1的含量.结果:HE染色和Masson染色显示模型组大鼠心肌细胞排列紊乱,丧失正常结构,心肌间质出现大量炎细胞浸润,胶原过度沉积,纤维明显增生;而芪参颗粒与假手术组排列整齐,细胞形态基本无变化,几乎没有沉积的胶原蛋白.RT-PCR和Western blotting显示模型组p47phox、RAC1在mRNA与蛋白层面显著增高;而芪参颗粒可显著降低p47 phox、RAC1的含量.结论:芪参颗粒能显著改善左冠脉结扎诱导诱导的大鼠心力衰竭,其机制可能与抑制p47phox、RAC1诱导的氧化应激效应有关.